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SRX4082260: GSM3141460: dek3arp6_27_T8_rep2; Arabidopsis thaliana; RNA-Seq
4 ILLUMINA (NextSeq 500) runs: 15.2M spots, 2.3G bases, 831.5Mb downloads

Submitted by: NCBI (GEO)
Study: Genome-wide analysis of transcription in dek3 mutants and DEK3-over expressors [2 temperatures at 2 time points]
show Abstracthide Abstract
The decision of whether to grow and proliferate or to restrict growth and develop resilience to stress is a key biological trade-off. Multiple tumour suppressors have roles in suppressing growth and proliferation when conditions are unfavourable, and aggressive tumours show re-wiring of the metabolic pathways and removal of these restraints on growth. In plants, constitutive growth results in increased sensitivity to environmental stress. However, the underlying mechanisms controlling this decision are not well understood. We used temperature as a cue to discover regulators of this process in plants, as it both enhances growth and development rates within a specific range, and is also a stress at extremes. We find the conserved chromatin protein DEK plays a central role in balancing the response between growth and arrest in Arabidopsis, and it does this via H2A.Z-nucleosomes. DEK target genes show two distinct categories of chromatin architecture, and these predict induction or repression by DEK. We show that these chromatin signatures of DEK target genes might be conserved in human cells, suggesting that DEK may act through a fundamental evolutionarily conserved mechanism to control the balance between growth and arrest in plants and animals. Overall design: RNA-seq were generated for seedlings in different temperature at 2 time points. ZT0 time point just before the lights are on (End of the night); ZT8-just before the light are off(end of the day)
Sample: dek3arp6_27_T8_rep2
SAMN09207740 • SRS3299476 • All experiments • All runs
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was isolated from 30 mg of grinded seedlings using the MagMAX-96 Total RNA Isolation kit (Ambion, AM1830), following the manufacturer's instructions. RNA quality and integrity was assessed on the Agilent 2200 TapeStation. Library preparation was performed using 1 ug of high-integrity total RNA (RIN>8) using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (NEB, cat. No. NEB #E7420). Libraries were sequenced in-house on an Illumina NextGen 500 sequencer using paired-end sequencing of 75 bp in length.
Experiment attributes:
GEO Accession: GSM3141460
Links:
Runs: 4 runs, 15.2M spots, 2.3G bases, 831.5Mb
Run# of Spots# of BasesSizePublished
SRR71640753,815,039575.5M207.8Mb2024-05-01
SRR71640763,717,249560.8M203.8Mb2024-05-01
SRR71640773,848,527580.7M210Mb2024-05-01
SRR71640783,833,419578.4M210Mb2024-05-01

ID:
5567821

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